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KMID : 0613820030130020214
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Journal of Life Science
2003 Volume.13 No. 2 p.214 ~ p.222
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Characterization of biopolymer produced by Bacillus coagulans DL-1 and optimization of its production
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Kim Ji-Mo
Son Chang-Woo
Seo Hyung-Phil
Moon Byung-Joo
Do Sung-Kook
Lee Jin-Woo
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Abstract
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The microorganism isolated from soil was identified as Bacillus coagulans by morphological and biochemical analyses and API-50CH/B kit, which was an identification kit for Bacillus species, and named as B. coagulans DL-1. It produced an extracellular biopolymer. Maximum production of biopolymer was 5.00 ±0.15 g/ℓ in a 7 ℓ bioreactor with an aeration rate of 1.0 vvm and an agitation speed of 500 rpm when concentrations of glucose and yeast as the optimal carbon and nitrogen sources were 2.0% (w/v) and 0.25% (w/v), which were optimized with a flask scale. Gas chromatographic analysis showed that the biopolymer producded by B. coagulans DL-1 consisted of glucose and rhanmose and their molar ratios was about 9 : 1. Its average molecular weight was 2.80 ×105 with gel permeation chromatographic (GPC) analysis.
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KEYWORD
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Extracellular polysaccharide, Bacillus coagulans, gas chromatography, molecular weight
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